Purification
This section covers the series of operations immediately following the cell culture, lysate or refold clarification process. The first of these unit operations is a capture where the product protein is typically bound to an affinity chromatography resin and then recovered by elution with a buffer solution. Once recovered, the protein solution is further purified and concentrated through filtration or additional chromatography steps until the final solution is ready for final fill operations.
The primary goals for successful protein processing are:
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Ultrafiltration (Tangential Flow Filtration) steps are used to reduce batch volumes and exchange buffers for efficient column operation or final formulation. Sequential capture, purification and polishing columns remove impurities to achieve specified product purity with high product recovery. Efficient process design maximizes the difference between product and impurity binding.
Overview
Integrated Solutions
| Chromatography | Tangential Flow Filtration |
Media | Membranes |
Column | Filters |
Systems | Systems |
Noticias sobre productos y lanzamientos
- Millipore Receives Award for Supply Consistency at Amgen Supplier Forum
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- Millipore Opens New Bioprocess Manufacturing Facility in Massachusetts
- Millipore presenta el biorreactor de un solo uso Mobius CellReady 3L en la reunión de la ESACT
Productos presentados
