Product Reference
Effect of Water Purity and Addition of Common Water Contaminants on Growth of Cells in Serum-Free Media
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| Author |
J. Mather, F. Kaczarowski, R. Gabler, F. Wilkins |
| Citation Information |
Biotechniques, 4:56-63 (1986) |
| Keywords |
WaterlineClubReference, cell, culture, RP222, media, cadmium, humic acid, distillation, UF, ultrafiltration, mammalian, serum, Cell culture, HPLC, TOC, Milli-Q |
| Applications |
Cell culture |
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Abstract
The effect of water quality on serum-free cell growth has been investigated for 3 cell lines. Water from 3 sources was used to make-up nutrient media. Tap water that was processed via a sequence of purification steps (Milli-Q) always gave the best results than distilled water in terms of cell numbers. Trace organic contaminants in the water appear to be more important than trace inorganics. Selected organic and inorganic contaminants were also added in known amounts to high purity water to demonstrate that specific water contaminants can affect cell growth. Small amounts of cadmium had the most marked effect on cell growth compared to controls, while other inorganics tested (magnesium, copper, lead) had variable effects. Of the organics tested, humic acid decreased growth the most.
