Product Reference
Comparison of Milli-Q PF Plus Water to DEPC-Treated Water in the Preparation and Analysis of RNA
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| Author |
Y. Huang, P. Leblanc, V. Apostolou et al. |
| Citation Information |
RP255;Biotechniques, 19:656-661 (1995) |
| Keywords |
WaterlineClubReference, RNAse, ribonuclease, nucleases, RNA, northern, southern, PCR, RNase-free, DEPC, diethylpyrocarbonate, Nuclease-free applications, Northern Blotting, Elix, Milli-Q, BioPak |
| Applications |
Molecular Biology, Nuclease-free |
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Abstract
The Milli-Q PF Plus water polishing system is equipped with high-purity ion and organic removal media and a capillary fiber ultrafiltration device. The system produces ultrapure water practically free of ribonuclease contamination? The necessity for DEPC treated solutions in RNA molecular biology procedures was tested ny preparing RNA from a variety of tissues and tissue-cultured cells using either DEPC-treated, autoclaved solutions or pure Milli-Q PF water dispensed directly from the system. RNA stability at 37°C was examined for various times using rabbit lung RNA in either DEPC-treated water or Milli-Q PF water. Intact RNA was detected after 6 hours in total RNA and by Northern Blots hybridized with Fibronectin? There was no significant difference in RNA degradation between DEPC-treated water and Milli-Q PF water. We conclude that Milli-Q PF water is an acceptable substitute for DEPC-treated water for the preparation of RNA and Northern Blot analysis.
