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The Y-box-binding protein, YB1, is a potential negative regulator of the p53 tumor suppressor.
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| Author |
Lasham, Annette, et al. |
| Citation Information |
J. Biol. Chem., 278: 35516-23 (2003), : (2003) |
| Applications |
Immunoblotting (Western) |
| Related Products |
06-499 |
| Pub Med ID |
12835324 |
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Abstract
The p53 tumor suppressor plays a major role in preventing tumor development by transactivating genes to remove or repair potentially tumorigenic cells. Here we show that the Y-box-binding protein, YB1, acts as a negative regulator of p53. Using reporter assays we show that YB1 represses transcription of the p53 promoter in a sequence-specific manner. We also show that YB1 reduces endogenous levels of p53, which in turn reduces p53 activity. Conversely, inhibiting YB1 in a variety of tumor cell lines induces p53 activity, resulting in significant apoptosis via a p53-dependent pathway. These data suggest that YB1 may, in some situations, protect cells from p53-mediated apoptosis, indicating that YB1 may be a good target for the development of new therapeutics.
