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Coordination of PIC assembly and chromatin remodeling during differentiation-induced gene activation.

Author	Evi Soutoglou, Iannis Talianidis
Citation Information	Science (New York, N.Y.), 295:1901-4 (2002)
Keywords	Acetylation, Acetyltransferases, Caco-2 Cells, Cell Differentiation, Chromatin, Enterocytes, Gene Expression Regulation, Histone Acetyltransferases, Histones, Humans, Nucleosomes, Phosphorylation, Promoter Regions, Genetic, Protein Binding, RNA Polymerase II, Saccharomyces cerevisiae Proteins, TATA Box, Transcription Factors, Transcription, Genetic, Transcriptional Activation, alpha 1-Antitrypsin
Applications	Chromatin Immunoprecipitation
Related Products	06-599
Pub Med ID	11884757

We analyzed the ordered recruitment of factors to the human alpha1 antitrypsin promoter around the initial activation of the gene during enterocyte differentiation. We found that a complete preinitiation complex, including phosphorylated RNA pol II, was assembled at the promoter long before transcriptional activation. The histone acetyltransferases CBP and P/CAF were recruited subsequently, but local histone hyperacetylation was delayed. After transient recruitment of the human Brahma homolog hBrm, remodeling of the neighboring nucleosome coincided with transcription initiation. The results suggest that, at this promoter, chromatin reconfiguration is a defining step of the initiation process, acting after the assembly of the Pol II machinery.