Content Loading...
Content Loading...
Product Reference
The Na,K-ATPase beta 2 subunit is expressed in rat brain and copurifies with Na,K-ATPase activity.
| |
|
| Author |
Shyjan, A W, et al. |
| Citation Information |
J. Biol. Chem., 265: 5166-9 (1990), : (1990) |
| Related Products |
06-171, 12-144 |
| Pub Med ID |
2156863 |
| |
|
Abstract
We have used a cloned fusion protein as antigen to generate an antiserum specific for the rat Na,K-ATPase beta 2 subunit. Utilizing this antiserum, we analyzed some of the structural features and tissue distribution of the beta 2 subunit. Treatment of a rat brain microsomal membrane fraction with N-glycanase F revealed that the beta 2 subunit is composed of an approximately 32 kDa core protein and at least two N-linked carbohydrate chains. The beta 2 subunit also was found to copurify with ouabain-inhibitable Na,K-ATPase activity from rat brain. Western blot analysis of rat tissue microsomes showed that beta 2 subunits were expressed in brain, pineal gland, and thymus. However, no beta 2 subunits were detected in kidney, heart, spleen, liver, mammary gland, or lung. These results suggest that the beta 2 subunit is a functional component of the rat brain Na,K-ATPase. The restricted tissue distribution of beta 2 subunits may reflect important differences in the functions of individual beta subunit isoforms.
