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In vitro differentiation of size-sieved stem cells into electrically active neural cells.
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| Author |
Shih-Chieh Hung, Henrich Cheng, Chien-Yuan Pan, May J Tsai, Lung-Sen Kao, Hsiao-Li Ma |
| Citation Information |
Stem cells (Dayton, Ohio), 20:522-9 (2002) |
| Keywords |
Antineoplastic Agents, Blotting, Western, Calcium, Cell Culture Techniques, Cell Differentiation, Cell Size, Culture Media, Fluorescent Antibody Technique, Hematopoietic Stem Cells, Humans, Membrane Potentials, Mercaptoethanol, Nerve Tissue Proteins, Neurons, Patch-Clamp Techniques, Tretinoin |
| Related Products |
MAB342, AP124P, AP132P |
| Pub Med ID |
12456960 |
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Abstract
Size-sieved stem (SS) cells isolated from human bone marrow and propagated in vitro are a population of cells with consistent marker typing, and can form bone, fat, and cartilage. In this experiment, we demonstrated that SS cells could be induced to differentiate into neural cells under experimental cell culture conditions. Five hours after exposure to antioxidant agents (beta-mercaptoethanol +/- retinoic acid) in serum-free conditions, SS cells expressed the protein for nestin, neuron-specific enolase (NSE), neuron-specific nuclear protein (NeuN), and neuron-specific tubulin-1 (TuJ-1), and the mRNA for NSE and Tau. Immunofluorescence showed that almost all the cells (>98%) expressed NeuN and TuJ-1. After 5 days of beta-mercaptoethanol treatment, the SS cells expressed neurofilament high protein but not mitogen-activated protein-2, glial filament acidic protein, and galactocerebroside. For such long-term-treated cells, voltage-sensitive ionic current could be detected by electrophysiological recording, and the intracellular calcium ion, Ca(2+), concentration can be elevated by high potassium (K(+)) buffer and glutamate. These findings suggest that SS cells may be an alternative source of undifferentiated cells for cell therapy and gene therapy in neural dysfunction.
