Millipore Applications Bibliography |
 | | |
Rapid assay of poly(ADP-ribose) glycohydrolase. | ||
| Authors: | Ménard, L and Poirier, G G | |
| Citation: | Biochem. Cell Biol., 65: 668-73 (1987) | |
| Pub Med ID: | 3325077 | |
| Year: | 1987 | |
| Abstract: | We have developed a rapid, highly reproducible assay to determine poly(ADP-ribose) glycohydrolase activity which measures directly the appearance of the reaction product. We also analysed the majority of different techniques which are used to determine poly(ADP-ribose) glycohydrolase activity and found that the apparent activity can vary extensively depending on the method used. Thin-layer chromatography using PEI-F-cellulose was the only method which evaluated directly the specific release of ADP-ribose; by comparison with this method, the other procedures gave an over- or under-estimation of 2- to 10-fold of the enzymatic activity. A rapid method of affinity chromatography has also been developed to synthesize and purify in high yield poly(ADP-ribose) (35% conversion of 1 mM NAD to poly(ADP-ribose)). | |
Please note: This information is provided to you for technical reference. A reprint of the document cited is available from the publisher or from your local library and is not available from Millipore. For further assistance, please contact Millipore's Technical Services.