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Product Reference
Rapid assay of poly(ADP-ribose) glycohydrolase.
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| Author |
Ménard, L and Poirier, G G |
| Citation Information |
Biochem. Cell Biol., 65: 668-73 (1987), : (1987) |
| Related Products |
MAB3192 |
| Pub Med ID |
3325077 |
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Abstract
We have developed a rapid, highly reproducible assay to determine poly(ADP-ribose) glycohydrolase activity which measures directly the appearance of the reaction product. We also analysed the majority of different techniques which are used to determine poly(ADP-ribose) glycohydrolase activity and found that the apparent activity can vary extensively depending on the method used. Thin-layer chromatography using PEI-F-cellulose was the only method which evaluated directly the specific release of ADP-ribose; by comparison with this method, the other procedures gave an over- or under-estimation of 2- to 10-fold of the enzymatic activity. A rapid method of affinity chromatography has also been developed to synthesize and purify in high yield poly(ADP-ribose) (35% conversion of 1 mM NAD to poly(ADP-ribose)).
