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Product Reference
Suppression of endogenous avidin-binding activity in tissues and its relevance to biotin-avidin detection systems.
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| Author |
Wood, G S and Warnke, R |
| Citation Information |
J. Histochem. Cytochem., 29: 1196-204 (1981), : (1981) |
| Related Products |
MAB377B |
| Pub Med ID |
7028859 |
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Abstract
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions, and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.
