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SynCAMs organize synapses through heterophilic adhesion.

Author	Adam I Fogel, Michael R Akins, Alexander J Krupp, Massimiliano Stagi, Valentin Stein, Thomas Biederer
Citation Information	The Journal of neuroscience : the official journal of the Society for Neuroscience, 27:12516-30 (2007)
Keywords	Animals, Cell Adhesion, Cell Adhesion Molecules, Neuronal, Cell Differentiation, Cell Line, Cells, Cultured, Coculture Techniques, Hippocampus, Humans, Macromolecular Substances, Mice, Neural Pathways, Presynaptic Terminals, Protein Isoforms, Rats, Rats, Sprague-Dawley, Synapses, Synaptic Membranes, Synaptic Transmission, Tumor Suppressor Proteins
Related Products	MAB377
Pub Med ID	18003830

Synapses are asymmetric cell junctions with precisely juxtaposed presynaptic and postsynaptic sides. Transsynaptic adhesion complexes are thought to organize developing synapses. The molecular composition of these complexes, however, remains incompletely understood, precluding us from understanding how adhesion across the synaptic cleft guides synapse development. Here, we define two immunoglobulin superfamily members, SynCAM 1 and 2, that are expressed in neurons in the developing brain and localize to excitatory and inhibitory synapses. They function as cell adhesion molecules and assemble with each other across the synaptic cleft into a specific, transsynaptic SynCAM 1/2 complex. Additionally, SynCAM 1 and 2 promote functional synapses as they increase the number of active presynaptic terminals and enhance excitatory neurotransmission. The interaction of SynCAM 1 and 2 is affected by glycosylation, indicating regulation of this adhesion complex by posttranslational modification. The SynCAM 1/2 complex is representative for the highly defined adhesive patterns of this protein family, the four members of which are expressed in neurons in divergent expression profiles. SynCAMs 1, 2, and 3 each can bind themselves, yet preferentially assemble into specific, heterophilic complexes as shown for the synaptic SynCAM 1/2 interaction and a second complex comprising SynCAM 3 and 4. Our results define SynCAM proteins as components of novel heterophilic transsynaptic adhesion complexes that set up asymmetric interactions, with SynCAM proteins contributing to synapse organization and function.