Product Reference
Impact of purified water quality on molecular biology experiments.
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| Author |
S. Mabic and I. Kano |
| Citation Information |
Clin. Chem. Lab. Med., 41: 486-91 (2003), : (2003) |
| Keywords |
WaterlineClubReference, LC-MS, HPLC, PCR, Elix, Milli-Q |
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| Pub Med ID |
12747591 |
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Abstract
Purified water is a reagent used in a variety of molecular
biology experiments, for sample and media preparation,
in mobile phases of liquid chromatography techniques,
and in rinsing steps. The combination of several
technologies in water purification systems allows delivering
high-purity water adapted to each application and
technique. Through a series of examples, the importance
of water quality on biotechnology experiments,
such as single nucleotide polymorphism (SNP) analysis
by denaturating HPLC, RNA preparation and PCR, is
presented. Results obtained on DNA mutation and single
nucleotide polymorphism analysis using the denaturating
HPLC (DHPLC) technique highlight the benefits
of organic removal by UV photooxidation process.
Comparative gel electrophoresis data show that ultrafiltration
is as efficient as diethylpyrocarbonate (DEPC)
treatment for suppressing RNase activity in water. Gel
electrophoresis and densitometry measurement also
point out the benefits of ultrafiltration to carry out reverse
transcriptase-polymerase chain reaction quantitatively.
