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Telomerase RNA inhibition using antisense oligonucleotide against human telomerase RNA linked to a 2',5'-oligoadenylate.

Author	Yasuko Kondo, Seiji Kondo
Citation Information	Methods in molecular biology (Clifton, N.J.), 405:97-112 (2007)
Keywords	Adenine Nucleotides, Apoptosis, Cell Line, Tumor, Cell Survival, Drug Screening Assays, Antitumor, Humans, Molecular Biology, Nucleic Acid Amplification Techniques, Oligonucleotides, Antisense, Oligoribonucleotides, RNA, Reverse Transcriptase Polymerase Chain Reaction, Telomerase, Telomere
Related Products	S7100
Pub Med ID	18369820

Telomerase, a ribonucleoprotein enzyme, is detected in the vast majority of cancers, including malignant gliomas, but not in most normal somatic cells. To inhibit telomerase function effectively, we have adopted the 2',5'-oligoadenylate (2-5A) antisense system. 2-5A is a mediator of one pathway of interferon actions by activating RNase L, resulting in single-stranded RNA cleavage. By linking 2-5A to an antisense oligonucleotide, RNase L degrades the targeted RNA specifically and effectively. Therefore, we have synthesized the antisense oligonucleotide against human telomerase RNA component (hTR) linked to 2-5A (2-5A-anti-hTR) and have demonstrated its antitumor effect on telomerase-positive cancer cells in vitro and in vivo.