Product Reference
Endotoxin-Free Water for Tissue Culture
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| Author |
E. Riché, J. Bôle and S. Mabic |
| Citation Information |
Lab Manager Magazine (2010) May, : (2010) |
| Keywords |
WaterlineClubReference, endotoxin-free applications, cell culture, Milli-Q, BioPak |
| Related Products |
ZRXQ003T0, ZRXQ003WW, ZRXQ005T0, ZRXQ005WW, ZRXQ010T0, ZRXQ010WW, ZRXQ015T0, ZRXQ015WW, CDUFBI001 |
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Water is a ubiquitous solvent used throughout cell isolation and culture. Therefore, water contamination may affect experimental outcomes. The use of ultrapure water is advisable for culturing of cells sensitive to environmental contaminants, such as endotoxins and bacteria. Cardiomycyte isolation experiments showed that using water contaminated with bacteria and endotoxins decreases cell viability, while using freshly produced ultrapure water dramatically increases it. Additionally, an ultrafiltration cartridge will efficiently remove large molecules that may be present in water.
