Product Reference
Rnase Undetectable in Water After Ultrafiltration
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| Author |
Joseph Plurad, Stephane Mabic |
| Citation Information |
Bioscience Technology 11; rp2350en00, : (2004) |
| Keywords |
WaterlineClubReference, Nuclease-free applications, Milli-Q, BioPak |
| Related Products |
Z00Q0V0WW, ZMQSP0D01, Z00QSV001, Z00QSV0WW, CDUFBI001, ZRXQ003T0, ZRXQ003WW, ZRXQ005T0, ZRXQ005WW, ZRXQ010T0, ZRXQ010WW, ZRXQ015T0, ZRXQ015WW |
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Although DEPC has been the standard preparation method for molecular biology-grade water, including water used in PCR, chemical treatment suffers from serious drawbacks. Contribution to TOC, the potential reversibility of Rnase inhibition, and lower pH may add to RNA degradation, resulting in the lowering of DA+NA amplificaiton in PCR. In addition, DEPC is toxic and has a measurable shelf-life. By removing RNase rather than merely inactivating it, ultrafiltration eliminates all the drawbacks of DEPC treatment. Ultrafiltration is a rapid, reliable and easy to use platform for quantitative, reproducible molecular biology experiments.
