Overview (TRTFF03 - Two - day course)
This two-day course examines in depth the theory of tangential flow filtration (TFF) as well as the latest techniques to develop a TFF process. During a practical laboratory session you will determine optimum operating conditions using a model feed stream on a laboratory system to perform concentration and cleaning steps. Then, through a real-life case study you will design a TFF process with specific product purity objectives.
Interactive case study
Using data from a real-life case study, participants will develop a process from scratch to the final purified product, including:
- Membrane and module selection.
- Operating parameters selection.
- Procedure determination.
- Mass balance and yield assessments.
- System design.
- Introduction to process economics.
What will you be able to do after attending this course?
- Develop a TFF process with your specific success criteria.
- Execute a screening and optimization protocol.
- Generate and collect data to design a new TFF process.
- Conduct robust and compliant TFF process scale-up.
What will you receive?
- A full set of course notes.
- Protocol and results of laboratory sessions.
- The solution from the workshop showing the design of a production scale TFF process.
- A certificate of attendance.
Who should attend?
This course is designed for R&D scientists and engineers who are responsible for developing and implementing production scale TFF processes. Participants must have completed the “Introduction to tangential flow filtration principles and operation” course or have equivalent experience.
Hands-on practical section
The laboratory exercise takes place under full operating conditions using a simulated feed stream. It covers:
- Membrane installation.
- Flushing.
- Feed flow determination.
- Optimum trans-membrane pressure (TMP) determination.
- Concentration and recovery.
- Cleaning and normalized water permeability (NWP) measurement.
Day 1 *9:00 – 18:00
- Introduction.
- TFF fundamentals.
- Brainstorming session on success criteria.
- Membrane selection.
- Module selection.
- Operating conditions determination.
- Laboratory session.
*Includes two coffee breaks and lunch both days.
Day 2 *8:30 – 17:00
- Laboratory session continued.
- Scale-up.
- System design.
- Review of selected TFF production processes in the biopharmaceutical industry.
- Visit to the TFF and chromatography manufacturing plant (if the course is held at EMD Millipore’s Molsheim facility).
- Case study.
- Conclusion.
Dates and Locations: Click here
This training can also be delivered on your site at a convenient time. Please contact your local EMD Millipore representative or send an email to accessknowledge@merckgroup.com to discuss this option.
Enrollment limited to 10 participants
Due to the interactive nature of this course, enrollment is limited to 10. We recommend early booking to ensure a place.
Ordering information
Catalogue number: TRTFF03
Price
Please contact your local EMD Millipore representative or send an email to accessknowledge@merckgroup.com for a quote.
Printable Course Description
