In Vitro Monoclonal Antibody Production

To meet the needs of our customers world wide, Millipore has invested in a cell factory facility that is dedicated to in vitro production of monoclonal antibodies. Our facility utilizes hollow fiber systems, CL units, and large profusion systems providing us with the capacity to produce as little as 10 mg to greater than 10 g of monoclonal antibody.

Ascites Production

•

Balb/c,nude, rats and other rodent strains are available upon request

•

Initial expansion, animal priming, and subclass typing

•

Number of animals is dependent on the required ascites volume:

• 20 mice (60–120 mL)

• 50 mice (150–300 mL)

• 100 mice (300–600 mL)

• 200 mice (600–1200 mL)

Purification Services

Monoclonal and polyclonal antibodies can be purified using:

•	Protein A or G chromatography
•	Ion Exchange chromatography
•	Immuno-affinity chromatography
•	Ammonium sulfate precipitation
•	Hydrophobic interaction chromatography (HIC)
•	Gel Filtration
•	Client dedicated columns

Antibody purification can be achieved even when the final yield is as low as 0.1mg of antibody.

Antibody Fragmentation Services

Fab and F(ab)’2 preparation and purification (10-50 mg antibody per process)

Bulk Capacity

Millipore has the manufacturing capacity to accommodate your bulk polyclonal and monoclonal antibody requirements. Our production facilities can produce in kilogram quantities,with sufficiently large lot sizes to lessen your manufacturing logistics. All manufacturing is performed according to cGMP guidelines, with corresponding documentation and quality oversights.

Protein Development

Antigen is obtained by synthesis of peptides and conjugation of these peptides to carrier proteins and production of recombinant protein. For synthesis of peptides, analysis of the protein sequence for immunogenic regions of the protein is essential. To generate antibodies to a peptide, the peptide must be covalently conjugated to a carrier protein prior to immunization. Protein expression is a subcomponent of gene expression. The genetic sequence coding for the target protein must be isolated and introduced into a suitable expression vector. The vector is placed in a suitable expression system (bacterial, insect, or mammalian cells, for example). The protein is produced by these cells and purified from the culture media in which the cells are grown.

The final purity depends upon the experiments, >70% pure for non-sensitive screening assays, >90% pure for immunological applications and >95% pure for receptor/ligand studies, bioassay studies or cellular assays.

For questions or comments please contact our specialized Custom Service Team at Millipore Corporation. Request a quote for Custom Services or Bulk Services

特殊&促销服务

a la Carte Lunch and Learn Seminars

查找MILLIPORE

查找就近的Millipore 办公室和应用专家。

联系我们

欢迎联系密理博。请点击各部门的相关链接或者“建议和意见”递交您的宝贵意见。