STEMCCA Lentivirus Reprogramming Kits

Featured Product:
STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

Product Highlight: STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit
STEMCCA reprogramming kits are lentiviral based reprogramming technologies that utilize a single polycistronic cassette encoding the four reprogramming factors, Oct4, Sox2, Klf4 and c-Myc, termed "Stem Cell Cassette (STEMCCA technology)" to efficiently reprogram mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs) into iPS cells. The use of a single lentiviral vector instead of four separate vectors for the derivation of iPS cells significantly reduces the risks of insertional mutagenesis and viral reactivation and is a step towards safer utilization of iPS technology for clinical therapies.
EMD Millipore’s STEMCCA lentivirus reprogramming kits make it easier than ever to obtain and work with induced pluripotent stem (iPS) cells. Unlike traditional iPS cell generation which requires simultaneous co-infection by four separate expression vectors, the STEMCCA kits use a single polycistronic lentiviral vector to improve efficiency and reduce the number of viral integrations.

STEMCCA Advantages:


FibroGRO Xeno-Free Human Foreskin Fibroblasts (HFF) are derived from normal human foreskin and have been isolated and propagated under xeno-free conditions, proliferating rapidly in FibroGRO LS (low serum) Complete Medium or xeno-free medium. Rapid proliferation of HFF enables efficient reprogramming of the cells to iPS cells. FibroGRO Xeno-Free Human Foreskin Fibroblasts have been tested and validated to generate iPS cells using STEMCCA Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit.