STEMCCA Lentivirus
Reprogramming Kits

Efficient iPS Cell Generation with a Single Vector

Millipore’s new STEMCCA lentivirus reprogramming kits make it easier than ever to obtain and work with induced pluripotent stem (iPS) cells. Unlike traditional iPS cell generation which requires simultaneous co-infection by four separate expression vectors, the STEMCCA kits use a single polycistronic lentiviral vector to improve efficiency and reduce the number of viral integrations.





Figure 1. (above) The STEMCCA vector is comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM), separated by the self-cleaving 2A peptide and IRES sequences driven by the EF-1α constitutive promoter1,2. It is also available with flanking LoxP sites incorporated for Cre-mediated excision of the exogenous reprogramming transgenes.

STEMCCA Advantages

• Efficient: uses a single vector with four transcription factors rather than co-transducing four separate expression vectors
• Minimizes viral integrations: single vector reduces the risks of insertional mutagenesis and viral reactivation
• Excisable: Cre/LoxP-regulated version enables removal of reprogramming transgenes
• Ready to use: convenient, pre-packaged lentiviral particles provided with detailed protocols

Ordering Information

1. 1. Sommer CA, et al. iPS cell generation using a single lentiviral stem cell cassette. Stem Cells. 2009 Mar;27(3): 543-9.
2. 2. Sommer CA, et al. Excision of Reprogramming Transgenes Improves the Differentiation Potential of iPS Cells Generated with a Single Excisable Vector. Stem Cells. 2010 Jan;28(1): 64-74.

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