| Cell-based assays are gaining increasing importance in pre-screening of compounds to qualify target leads for drug discovery. These programs are increasingly focused on incorporating functional cell-based assays in the pre-screening stages of lead compounds. Many drugs under development are directed at altering the functional properties of cancer cells such as migration, chemotaxis and invasion. Cells that interact with these cancer cells, such as the Human Umbilical Vein Endothelial Cells (HUVEC), in invasive processes such as angiogenesis are also of interest drug targets. Development of HTS cell-based assays that are designed to be able to measure effects of lead compounds on such functional processes pose a challenge. We have developed a 96 well MultiScreen-MIC plate to support these HTS cell-based assays. The data presented in this poster indicate that MultiScreen-MIC plates are useful for anti-cancer drug HTS and can serve as an alternative to existing, lower throughput methods. |
