Millipore Technical Publications |
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MultiScreen Permeability PlatesThe evaluation of the reproducibility of passive, transcellular drug permeability assays | ||
| Lit No: | AN1725EN00 | |
| Year: | 2002 | |
| Abstract The reproducibility and precision of a high throughput method based on a published method using a Millipore plate (MultiScreen Cat. MAPBMN310) for predicting passive, transcellular compound permeability was assessed. The permeability of six drugs (propranolol, methotrexate, warfarin, carbamazepine, furosemide, and testosterone) was measured on different days using five different lots of (MultiScreen) plates. In addition several protocol variations (incubation times, volumes, temperature, etc.) were tested to simulate the analytical variability that might be encountered from lab to lab and from operator to operator. Our results show that under typical conditions, this permeability assay is robust and generates reproducible data. We also observed that small protocol variations can have an effect on the apparent permeability rates of some drugs but in general the rank order (by log Pe) of the compounds is unaffected. Click the PDF above for the full document. | |
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