Millipore Technical Publications

Low Background Membrane for Fluorescent Protein Detection in Western Blotting

• We demonstrate the use of novel, low-fluorescence PVDF membrane (Immobilon™ FL) for western blotting applications.
• The background fluorescence of the membrane is about 10 times lower than that of other commercially available PVDF membranes, and at least 2 times lower than the fluorescence of nitrocellulose membranes.
• The use of Immobilon FL membrane does not require any changes in standard western blotting protocol, and the membrane is shown to be compatible with variety of blocking reagents and transfer buffers, as well as total protein blot stains.