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Millipore Technical Publications


  ds0126en00.pdf
Guava Cellgrowth Assay
For easy and sensitive cell proliferation analysis of primary cultures

Assessments out to 5 generations
The Guava Cell Growth Assay provides, at the single-cell level, proliferation assessments out to five generations. This kit is a powerful tool for assessing cell doubling time, verifying the health of cell cultures, or screening for compounds that affect cell growth.

Simple, non-radioactive protocol
The assay uses a well-characterized fluorescent dye, carboxyfluorescein diacetate succinimidyl ester (CFSE), which diffuses freely into cells and does not affect cellular function. For each round of cell division, the relative fluorescence intensity of the dye is decreased by half. A dead cell dye, PI, is also included.

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