| A panel of 80 anti-inflammatory, anti-neoplastic and anti-infective compounds were screened for their ability to cause cell death or apoptosis induction using
rapid and objective 96-well plate assays that measure
cell concentration and viability (Guava® ViaCount®) or
Annexin V binding to apoptotic and dead cells (Guava
Nexin®). The Guava PCA™-96, a novel benchtop microcapillary
cell analysis system, was used to acquire the
data for both assays. Compounds were characterized
for potency (IC50 or EC50), efficacy (percent killed or
percent positive for apoptosis), and specificity (which
cells were affected). Twenty-three drugs exhibited
minimal to substantial activity on both cell lines inducing
either apoptosis, cytotoxicity or both; four were effective
against only CHO cells and ten against only Jurkats.
Moreover, Guava ViaCount and Guava Nexin, both of
which detect apoptosis induction and cytotoxicity,
albeit in different ways, could be used in combination to
confirm the specific activity of the drugs or other compounds
as well as to identify potential false positives. |
