| The Guava PCA system allows you to perform
multiple assays to examine cells at various stages
in the progression of apoptosis. The Guava ViaCount®
assay can identify cells which have entered the apoptotic
pathway by differential permeability of nucleic
acid staining dyes, while providing an accurate cell
count and viability assessment. The Guava NexinTM
assay measures membrane changes associated with
apoptosis using Annexin V-PE, and 7-AAD to identify
dead cells. The Guava MultiCaspase assay tags activated
caspases within cells with a fluorescent inhibitor
(SR-VAD-FMK) and uses 7-AAD to identify dead cells.
The Guava TUNEL assay detects the fragmentation of
nuclear DNA to analyze cells committed to the apoptotic
pathway.We performed the Guava ViaCount,
Guava MultiCaspase, Guava Nexin and Guava TUNEL
assays on CHO-K1 cells prepared from a single induction
treatment to look at the differences in
quantifying apoptotic cells by the different methods.
Each assay is distinguishing apoptotic from nonapoptotic
cells; however, they are identifying cells at
different stages through the progression of apoptosis
with different markers of apoptosis. |
