Millipore Technical Publications |
 | TN- TN1075EN00 | |
IFN-γ ELISpot Assays on MultiScreen HA IFN-γ ELISpot Assays on MultiScreen HA | ||
| Lit No: | TN1075EN00 | |
| Year: | 2002 | |
| The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical Elispot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications. |
| Click the PDF above for the full document. |