Millipore Technical Publications |
 | UG-17-500 | |
Catch and Release v2.0 Reversible Immunoprecipitation SystemFor laboratory research use only. Not intended for human or animal diagnostic, therapeutic or other clinical uses. | ||
| Lit No.: | 17-500 | |
| Immunoprecipitation (IP) is a frequently used method to purify specific proteins from complex samples such as cell lysates or extracts. Traditional IP protocols use Protein A or Protein G coupled to an insoluble resin, such as agarose beads, to capture an antigen:antibody complex in solution. The complex is then “precipitated” by centrifugation. Limitations of traditional IP include sample handling and processing difficulties, the inability to release native antigen from the beads for functional assays and poor reproducibility and recovery due to multiple wash steps. Catch and Release overcomes many of the limitations associated with traditional IP. Its unique Spin-Column format was designed to make IP faster, simpler and more reproducible. Catch and Release enables the elution of the antigen:antibody complex without denaturation, while ensuring minimal contamination by non-specific proteins in the eluate. In addition, the convenient Spin-Column format of Catch and Release improves performance and makes higher throughput processing of samples possible. |
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