 | ProSep-A protein A affinity chromatography media provide high throughput purification for monoclonal antibodies combined with long lifetime and broad process flexibility(1)(2). ProSep-A, in common with all chromatography media, can exhibit instances of non-specific binding. Such non-specific binding is characterized as the binding of any non-immunoglobulin species present in the feed. In general, the levels of non-specific binding to ProSep-A are very low and the purity of the eluate can routinely exceed 98%. In some cases, a more precise form of "non-specific binding" has been reported. In these cases, rather than "general" non-specific binding the problem is due to a particular component of the feed that binds and co-elutes with the immunoglobulin. This note provides recommendations on how to minimize or eliminate non-specific binding and makes some general recommendations for cleaning ProSep-A media. |
