Millipore Technical Publications |
 | Brochure - 170-0706 | |
Kinease - Fluorescence Polarization Kinase Activity Assays | ||
| Lit Number: | 170-0706 | |
| Year: | 2007 | |
KinEASE advantages
Upstate® KinEASE from Millipore is a robust fluorescence polarization kinase assay that unites our expertise in monoclonal antibodies and kinase profiling to provide a high quality, robust, nonradioactive kinase assay compatible with 80 different kinases.
- No practical limits on ATP concentration, allowing for the identification of both competitive and allosteric inhibitors
- Robust signal-to-noise ratio with less than 10% substrate conversion, enabling linear rate determination
- Red or green fluorescent labeled tracers available, featuring FarRed dye to avoid compound interference, eliminating the concern of false negatives
- Ratiometric readout, reducing errors caused by experimental noise, ensuring correct, reproducible data
- One platform to measure the activity of 80 kinases, allowing for relative data comparison
- The three phospho-specific antibodies used in this assay have been validated and optimized, saving you time
Download the PDF for complete information.