Millipore Technical Publications |
 | AN- AB3355EN00 | |
The Direct Detect™ Biomolecular Quantitation SystemExploits Membrane Technology to Enable Accurate, Infrared-based Detection. | ||
| Lit No: | AB3355EN00 | |
| Year: | 2012 | |
| Abstract The Direct Detect™ system, an infrared (IR)-based spectrometry system, represents an innovation in biomolecular quantitation. The key to this advance lies in a new membrane technology for preparing and presenting aqueous biological samples to make them compatible with infrared analysis. Built upon EMD Millipore ’s extensive experience in membrane technology, it employs a hydrophilic polytetrafluoroethylene (PTFE) membrane that is designed to be transparent in most of the infrared spectral region and enables application of biomolecule solutions directly onto the membrane. The Direct Detect™ system has been optimized for detection and quantitation of proteins. By measuring amide bonds in protein chains, the system accurately determines an intrinsic component of every protein without relying on amino acid composition, dye binding properties or redox potential. Click the PDF above for the full document. | |
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