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Millipore Technical Publications


AN1045EN00.pdf
AN- AN1045EN00

Clarification and Recovery of Recombinant Proteins using a Cascade Tangential Flow Filtration (TFF) System

Lit No:AN1045EN00
Year:2004


A cascade TFF system is defined as a two stage TFF system. The first stage clarifies the feed stock using a microfiltration membrane (MF) while the second stage concentrates the permeate from the first stage using an ultrafiltration membrane (UF). The permeate from the second stage is recycled for use as the diafiltration medium for the first stage. The product of interest is in the retentate from the second stage.

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