Millipore Technical Publications |
 | TN- AN1066EN00 | |
Improved Method for Adenovirus and Lentivirus Purification using the Fast-Trap Virus Purification and Concentration Kit | ||
| Lit No: | AN1066EN00 | |
| Year: | 2008 | |
| Highly purified virus is necessary for many viral vector applications such as vaccine production and genetic modification. Crude virus preparations often contain cellular debris and proteins from culture media that are toxic to target cells and can cause immunogenic reactions in vivo. Conventional virus purification methods based on sucrose or cesium gradient ultracentrifugation are time-consuming, difficult, and require access to special instrumentation. These methods also often result in low virus recovery. Here we report a fast and easy method for adenovirus and lentivirus purification using the Millipore Fast-Trap Virus Purification and Concentration Kit. Both adenovirus (Cat. No.FTAV 000 03) and lentivirus (Cat. No. FTLV 000 03) kits contain the necessary reagents and filter devices to accommodate the entire virus purification workflow (Figure 1). The purification is membrane-based and utilizes a closed vacuumdriven device. High recoveries of purified viable viral particles are typically achieved in under two hours. Click the PDF above for the full document. | |
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