 | Though microporous filtration is the method of choice to prepare and minimize contamination of cell culture media and reagents, there still remains some concern that the filtration step will remove valuable factors and/or add deleterious components to the growth media. This concern is especially true for stem cell culture where factors can be expensive, the cells can be very sensitive to additives, and culture durations may be very long. Here we show that a PES membranecontaining Stericup filter, even after many multiple filtration steps, provides a worry-free alternative for the pluripotent expansion of embryonic stem cells.
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