Millipore Technical Publications |
 | TN- AN1071EN00 | |
ESGRO Complete Products for Mouse Embryonic Stem Cell Culture in Serum-Free and Feeder-Free Conditions | ||
| Lit No: | AN1071EN00 | |
| Year: | 2008 | |
| A major challenge in harnessing the potential of embryonic stem (ES) cells for both in vitro and in vivo use is the controlled and reproducible self-renewal of ES cells in the laboratory.Currently, the routine culture of mouse ES cells largely relies on the addition of serum and in some cases the presence of a feeder layer. Serum is an inherently heterogeneous, undefined product that has batch to batch variability, while the use of feeder cells is cumbersome and adds extra variability. A fully defined and easy to use culture system would lead to more systematic and uniform approaches to large scale screening projects. We have developed a fully defined, serum and feeder free system based on the work of Ying et al.1 that allows for the efficient self-renewal of mouse ES cells. This system enables the researcher to successfully passage, freeze and thaw ES cells in a serum free environment while maintaining their pluripotency. Unlike other systems, ES cells grow efficiently at clonal density making this system suitable for transfection and targeting experiments. Here we describe observations on ES cell growth characteristic of this system and the differences with those cells grown in serum. Click the PDF above for the full document. | |
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