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Millipore Technical Publications


an1073en00.pdf
TN- an1073en00

ESGRO Complete Clonal Grade Medium: Serum-Free Medium for Mouse Embryonic Stem Cell Culture

Lit No:an1073en00
Year:2008


ESGRO Complete Clonal Grade Medium is a fully defined,serum-free media for mouse ES cell culture.
It is equivalent to serum-containing media for the maintenance of murine embryonic stem cells in the
pluripotent state, even in feederfree culture. ESGRO Complete Clonal Grade Medium is suitable for all
standard transgenic protocols from single-cell cloning to the efficient generation of chimeric animals, and
thus can replace the need for serum-containing media for multiple mouse ES cell applications. Also noteworthy
are the utility of two additional reagents for serum-free culture of murine ES cells, ESGRO Complete Accutase for
cell dissociation and ESGRO Complete Freezing Medium for serum-free cryopreservation.


Click the PDF above for the full document.