Millipore Technical Publications |
 | AN- AN1234EN00EM | |
Controlling Interfering Antibodies in Diagnostic Assays | ||
| Lit No: | AN1234EN00EM | |
| Year: | 2012 | |
| It is well recognized that immunoassays, particularly in sandwich formats, are subject to interference from a range of endogenous antibodies. These include anti-animal antibodies, rheumatoid factor, other auto-antibodies and heterophilic antibodies. They can be from all classes (IgG, IgA, IgM or IgE) and can bind to antibodies from other species, as well as other cell fractions or assay components. These endogenous antibodies can create both false positive and false negative results by changing the binding ability of the target analyte or assay antibodies, giving an analytical response that is inconsistent with a patient’s true clinical condition. It can be difficult to recognize and resolve these spurious results. Moreover, there are numerous reports where patients were incorrectly diagnosed and unnecessarily or incorrectly treated. Click the PDF above for the full document. | |
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