Millipore Technical Publications |
 | TN- AN1237EN00 | |
A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions | ||
| Lit No: | AN1237EN00 | |
| Year: | 2008 | |
| Human embryonic stem cells (hESCs) hold great promise for therapeutic applications. However, their use in regenerative medicine is hindered by the presence of non-human and/or undefined components typically included in the maintenance media. The development of HEScGRO medium is a step towards establishing humanized and defined conditions for hESC growth. For this study, we performed direct, side-byside comparisons between the growth characteristics of hESCs in HEScGRO medium and traditional serum replacement conditions. Short- and long-term growth parameters were examined in detail. Specifically, general morphology and karyotype was followed, proliferation and apoptosis assays were performed, the expression of pluripotency markers by immunocytochemistry and FACS analysis was investigated, and the differentiation potential was analyzed. Culture of hESCs in HEScGRO medium maintained the cells in a pluripotent state, and the characteristics of these hESCs were similar to those grown in serum replacement media conditions. HESCs grown in humanized and defined media such as HEScGRO medium will be useful for basic research and also, significantly, for downstream applications that include cell-based therapies. Click the PDF above for the full document. | |
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