Millipore Technical Publications |
 | AN- AN1257EN00 | |
CellPrime Recombinant Human Transferrin as an Animal-Free Alternative to Animal-Derived Transferrin or Iron Supplementation in Cell Culture Applications | ||
| Lit No: | AN1257EN00 | |
| Year: | 2008 | |
| Iron is essential for key cellular processes, including cell growth and DNA synthesis. Iron is typically transported in vivo by transferrin, which regulates efficient delivery of iron to cells. In the biopharmaceutical setting, antibody- producing cell lines such as CHO and NS0 require an iron source such as transferrin to attain optimal cell growth and protein production. Traditionally transferrin has been supplied in the form of serum-derived purified human transferrin (hTf) or bovine transferrin (bTf). Current regulatory pressure to remove animal-derived products from biopharmaceutical manufacturing has led researchers to explore alternate means to animal-derived transferrin. As an alternative, inorganic iron salts have been used to supply iron to mammalian cells. However, to provide high density cell cultures with sufficient iron, elevated concentrations of iron salts are required that utilize low affinity non-transferrin receptor pathways. This can negatively impact cell growth due to the formation of free radicals and oxidative stress from the unbound ferric or ferrous irons. Click the PDF above for the full document. | |
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