Millipore Technical Publications |
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MultiScreen Solubility Filter PlateQuantitative method to determine drug aqueous solubility: optimization and correlation to standard methods | ||
| Lit No: | AN1730EN00 | |
| Year: | 2003 | |
| Abstract Correlation to standard methodology, reproducibility, solvent (DMSO) effect, and drug recovery (non-specific binding, NSB) were determined for a high throughput aqueous solubility assay. Using a new, 96-well filter plate (MultiScreen Solubility filter plate, Millipore, Billerica, MA), and a protocol compatible with full automation, the aqueous solubility of thirty-two commercial compounds was quantitatively measured. Sample throughput allows for 4 plates a day with 32 samples per plate. Correlation with shake-flask values is reported. Day-to-day, plate-to-plate, and within plate assay variability was determined for a number of compounds whose solubility spans the assay’s analytical range (10–500 µM). Effects of incubation time and DMSO on aqueous solubility, as well as NSB, have also been evaluated. Click the PDF above for the full document. | |
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