Millipore Technical Publications |
 | AN- an1967en00 | |
Fluorescent Immunodetection Using the SNAP i.d. Protein Detection System | ||
| Lit No: | an1967en00 | |
| Year: | 2008 | |
| Recent advances in fluorescent dye chemistry and blotting membranes coupled with improvements in instrumentation have accelerated the application of fluorescence detection methodologies to western blotting. Immunodetection via fluorescently-labeled primary or secondary antibodies offers researchers several advantages over conventional, enzymatically-catalyzed chemiluminescent detection. These advantages include enhanced dynamic range, a greater degree of multiplexing, and the potential for more accurate quantitation of the immunoreactive species. Despite these advances, the overall work flow for immunodetection has remained largely unchanged and has, up until now, required 4 hours or more to complete The SNAP i.d. Protein Detection System has been developed to substantially shorten the time required for fluorescent immunodetection through the use of vacuum. All of the immunodetection steps, starting with blocking and proceeding through antibody incubations and washing, can be completed within 30 minutes. This is achieved without any additional reagent (e.g., fluorescent antibody) consumption. Sensitivity, background, and signal-to-noise ratios are the same or better than that of traditional immunodetection techniques. The SNAP i.d. system uses small volumes for antibody incubations and is compatible with either nitrocellulose or low fluorescence background Immobilon®-FL polyvinylidene difluoride (PVDF) blotting membranes. The SNAP i.d. Protein Detection System works with most blocking reagents and fluorescently-labeled secondary antibodies.. Click the PDF above for the full document. | |
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