Millipore Technical Publications |
 | TN- AN2634EN00 | |
Rapid Assessment of Size Changes Using the Scepter Cell Counter can be Predictive of Cell Death | ||
| Lit No: | AN2634EN00 | |
| Year: | 2010 | |
| The process of programmed cell death or apoptosis is an essential component in many biological processes and is therefore the focus of much research. Initial studies revealed that reduction in cell volume was an early morphological change during apoptosis1. Recent studies have shown that this cell shrinkage is not simply a passive consequence of other apoptotic events, but is a key driver of apoptotic signaling, mediated by the cell’s machinery for homeostatic regulation2-4.
Apoptotic enzymes, including caspases, and membrane depolarization events are activated by changes in intracellular ionic concentrations. The cell actively transports potassium, sodium, calcium, and/or chloride ions to enable apoptosis to occur, and these ionic fluxes cause cell volume changes during apoptosis4. The specific ions transported and the directionality of transport depends on the specific cell type and particular apoptotic stimulus.
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