Millipore Technical Publications |
 | TN- AN3311EN00 | |
Human PBMC Isolation and Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter | ||
| Lit No: | AN3311EN00 | |
| Year: | 2011 | |
| The Scepter cell counter combines the ease of automated instrumentation and the accuracy of impedance-based particle detection using the Coulter principle in a handheld format. The instrument uses a combination of analog and digital hardware for sensing, signal processing, data storage, and graphical display. The precision-made, consumable polymer sensor has a laser-drilled aperture in its cell sensing zone that enables the instrument to use the Coulter principle to discriminate cell diameter and volume at sub-micron and sub-picoliter resolution, respectively. Previous work has shown that, by using the new 40 μm aperture sensor, the Scepter cell counter was able to accurately and precisely count a much broader range of cell types, including small cells (< 6 μm in diameter) such as PBMC and red blood cells (RBC). This short report outlines a protocol for the isolation of PBMC from whole blood and subsequent sample analysis using the Scepter cell counter. Click the PDF above for the full document. | |
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