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Millipore Technical Publications


AN3313EN00eLit.pdfAN3313EN00eLit.pdf
TN- AN3313EN00

Counting Yeast Cells for Brewing and Wine Industries Can Be Facilitated by the Scepter™ 2.0 Handheld Automated Cell Counter

Lit No:AN3313EN00
Year:2012



The Scepter cell counter offers an easy-to-use alternative to current methods of yeast cell counting. With the Scepter cell counter, EMD Millipore has captured the ease of automated instrumentation and accuracy of impedance-based counting using the Coulter principle in an affordable, handheld format. The instrumentation has been collapsed into a device the size of a pipette and uses a combination of analog and digital hardware for sensing, signal processing, data storage, and graphical display in the form of a histogram. The 40 μm- and 60 μm-aperture sensors placed at the tip of the instrument are engineered with a microfabricated, sensing zone that enables discrimination by cell size and cell volume at sub-micron and sub-picoliter resolution, respectively. Here, we outline the method for using Scepter counting for the enumeration of yeast cells.

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