Millipore Technical Publications |
 | AN- AN3666EN00 | |
Rapid Analysis of Human Adipose-Derived Stem Cells and 3T3-L1 Differentiation Towards Adipocytes Using the Scepter™ 2.0 Cell Counter | ||
| Lit No: | AN3666EN00 | |
| Year: | 2012 | |
| This study outlines a method for tracking adipogenic
differentiation of ADSCs and 3T3-L1 cells and subsequent
sample analysis using the Scepter™ cell counter to
measure these changes. We have employed Scepter™
technology for determining cell size and volume, as well
as lipid vacuole staining using Oil Red O as a crossvalidation
histology screen to investigate the relationship
between cell differentiation and cell size changes.
Although the Scepter™ cell counter was intended
primarily as a cell counting device, we demonstrate how
this cell counter can also function as a reliable tool to
track phenotypic change.
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