Millipore Technical Publications |
 | AN- AN3772EN00 | |
Precise and Accurate Bead Counting Using the Scepter™ 2.0 Handheld Automated Cell Counter | ||
| Lit No: | AN3772EN00 | |
| Year: | 2012 | |
| Introduction With the Scepter™ cell counter, EMD Millipore has captured the ease of automated instrumentation and accuracy of impedance-based counting using the Coulter principle in an affordable, handheld format. The instrumentation has been collapsed into a device the size of a pipette, and uses a combination of analog and digital hardware for sensing, signal processing, data storage, and graphical display in the form of a histogram. The 40 μm- and 60 μm-aperture sensors placed at the tip of the instrument are engineered with a microfabricated, sensing zone that enables discrimination by bead size and bead volume at sub-micron and sub-picoliter resolution, respectively. Table 1 outlines the specifications for each sensor type. While the Scepter™ cell counter was initially optimized for cell counting, here we report that the device is also well suited for precise counting for beads of numerous types commonly employed in a wide range of biological applications. Click the PDF above for the full document. | |
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