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Millipore Technical Publications


AN3775EN00.pdfAN3775EN00.pdf
TN- AN3775EN00

Rapid Counting of Somatic Cells in Dairy Milk Using the Scepter 2.0 Cell Counter, Following Spin-Wash Sample Preparation

Lit No:AN3775EN00
Year:2011



The Scepter handheld, automated cell counter provides a rapid, reliable alternative to traditional SCC analysis. The device combines the ease of automated instrumentation and the accuracy of the Coulter principle of impedance-based particle detection in an affordable, handheld format. The Scepter cell counter uses a combination of analog and digital hardware for sensing, signal processing, data storage, and graphical display. The precision-made, consumable polymer sensor has a laser-drilled aperture in its cell sensing zone that enables the instrument to use the Coulter principle to discriminate cell diameter and volume at submicron and sub-picoliter resolution, respectively.

The spin-wash protocol, when used in conjunction with the Scepter cell counter and 40 μm aperture sensor, enables rapid, precise somatic cell counting with results comparable to fluorescence-based platforms.

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