Millipore Technical Publications |
 | AN- AN4228EN00 | |
Scalability of the Mobius® CellReady Single-use Bioreactor Systems | ||
| Lit No: | AN4228EN00 | |
| Year: | 2012 | |
| Abstract The Mobius® CellReady single-use bioreactor systems are designed for mammalian cell growth and recombinant protein production. The Mobius® CellReady product offering includes bench-scale (3 L), small-scale (50 L) and pilot-scale (200 L) bioreactor systems spanning early process development through clinical batch production. To enable the successful scale-up of a biomanufacturing process, a number of factors critical to efficient cell growth, viability and protein production were considered and used to develop an optimized design. These include mixing efficiency, gas transfer capability, and an operating range of power input that minimizes cell shear. In this study, several key design parameters, such as oxygen mass transfer coefficient (kLa), power per unit volume, Reynolds number (Re), mixing time and tip speed were characterized for the three different sized single use bioreactor process containers. Based on these data, CHO cells were cultured in each of these bioreactor systems maintaining equivalent power per unit volume as the primary scaling parameter. These data provide evidence of cell culture scalability across the entire Mobius® CellReady single-use bioreactor platform. Click the PDF above for the full document. | |
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