Millipore Technical Publications |
 | AN- AN4775EN00 | |
Long-term, live cell imaging of host-pathogen interactions using the CellASIC™ ONIX System | ||
| Lit No: | AN4775EN00 | |
| Year: | 2012 | |
| The CellASIC™ ONIX Microfluidic System is well suited for host-pathogen studies by providing a stable, longterm culture environment for host cells (including primary cells) with controlled pathogen exposure. The continuous perfusion format and the ability to switch media solutions enable wash-out of pathogens from the chamber and subsequent monitoring of host cell response over many days. The enclosed small volume of the culture chamber also provides practical advantages for working with infectious agents during live cell imaging. The design of the microfluidic plates enables the dynamics of infection to be tracked using live cell imaging on standard inverted microscopes. Here, we demonstrate a host-pathogen experiment using human intestinal cells infected with engineered E. coli strains (Figure 1). Both an invasive and noninvasive bacterial strain were monitored for long-term infection with time-lapsed imaging up to 24 hours in the CellASIC™ M04S microfluidic plate. Click the PDF above for the full document | |
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