Millipore Technical Publications |
 | AN- AN5227EN00 | |
Automated live cell imaging of cell migration across a microfluidic-controlled chemoattractant gradient | ||
| Lit No: | AN5227EN00 | |
| Year: | 2013 | |
| In order to create a quantitatively defined diffusion gradient that is stable enough for long-term, live cell imaging over the course of days, we developed a microfluidic gradient plate. This plate, designed for use on the CellASIC™ ONIX Microfluidic Platform, enables precision-controlled chemoattractant diffusion across perfusion barriers to create a spatial gradient in the culture area. On the other end of each culture area, there are flow outlets. Together, the perfusion inlets and outlets form a continuous flow “infinite source/sink” that maintains a stable concentration gradient profile for days. The flexible format of the plate enables changes in gradient directionality, turning gradients on and off, and toggling between gradient and single solution exposure. Click the PDF above for the full document. | |
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