Millipore Technical Publications |
 | AN- AN5532EN00 | |
Demonstration of long-term culture and in-plate staining protocols using the CellASIC™ ONIX Microfluidic platform | ||
| Lit No: | AN5532EN00 | |
| Year: | 2013 | |
| The ability to analyze cell responses to perturbations over time greatly extends the precision and biological relevance of in vitro studies. However, there are technical challenges to successful long-term cell culture and imaging. Not only is it difficult to control the temperature and gas composition of the cell environment during the course of the experiment without impeding optical access to the cells, but it is also challenging to maintain sufficient nutrient supply and outflow of waste to keep cells healthy. The easy-to-use CellASIC™ ONIX Microfluidic Platform delivers precise control of perfusion, temperature and gas conditions, facilitating long-term cell culture and enabling automated, in-plate cytochemical analysis using minimal reagent volumes. Here, we demonstrate the long-term culture of multiple cell lines using automated perfusion protocols set up with intuitive CellASIC™ ONIX FG Software, comparing the results to those obtained using traditional chambered slides. Our results indicate that optimal experimental parameters, such as flow rates, flow duration, extracellular matrix, gas composition and medium change intervals, can vary greatly with respect to the particular cells being cultured. Click the PDF above for the full document. | |
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