Millipore Technical Publications |
 | AN- AN5533EN00 | |
Three-dimensional culture and assessment of drug-induced cell death using the CellASIC™ ONIX Microfluidic Platform | ||
| Lit No: | AN5533EN00 | |
| Year: | 2013 | |
| We used the CellASIC™ ONIX Microfluidic System, in conjunction with the M04S microfluidic plate, to achieve perfusion-based microenvironment control and study drug-induced cell death of 3D cultures of MCF7 cells in Matrigel®. The microfluidic chamber recreates the physiologic mass transport condition for optimized cell health. Four upstream fluidic channels allow controlled exposure of the cells to different solutions. The plate can also be cultured in a standard incubator using a dedicated gravity-driven flow channel. The cells are in contact with a #1.5 thickness (170 μm) optical glass surface, enabling high quality imaging using an inverted microscope. An integrated micro-incubator system delivers temperature and gas control to the microfluidic chambers. Click the PDF above for the full document. |
